Journal: Frontiers in Pharmacology

Article Title: Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway

doi: 10.3389/fphar.2025.1567762

Figure Lengend Snippet: TQ Ameliorates Oxidative Stress and Apoptosis in Rat Perforator Flaps After Ischemia-Reperfusion Injury. (A) Immunohistochemical staining images of SOD1 in the control and TQ groups (scale bar, 50 μm). (B) Quantitative analysis of SOD1 absorbance in the control and TQ groups. (C) Western blotting to detect the expression of oxidative stress-related proteins: eNOS, HO1, and SOD1 in flap tissues of the control and TQ groups. (D) Quantitative analysis of oxidative stress-related protein blots. (E) Immunohistochemical staining images of C-CASP3 in the control and TQ groups (scale bar, 50 μm). (F) Quantitative analysis of C-CASP3 absorbance in the control and TQ groups. (G) Western blotting to detect the expression of apoptosis-related proteins: Bax, C-CASP3, and Bcl-2 in flap tissues of the control and TQ groups. (H) Quantitative analysis of apoptosis-related protein blots. Significance: *p < 0.05 and **p < 0.01. Data are presented as mean ± SEM, with five rats per group.

Article Snippet: Anti-cadherin 5 is provided by Wuhan Boster Biological Technology, Ltd. Primary antibodies against GAPDH, HO1, SOD1, Bax, Bcl-2, GSDMD-N, and VEGF are sourced from The Proteintech Group (Chicago, USA).

Techniques: Immunohistochemical staining, Staining, Control, Western Blot, Expressing

Journal: Frontiers in Pharmacology

Article Title: Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway

doi: 10.3389/fphar.2025.1567762

Figure Lengend Snippet: EX527 Reverses the Inhibitory Effects of TQ on Pyroptosis, Oxidative Stress, and Apoptosis in Rat Perforator Flaps After Ischemia-Reperfusion Injury. (A) Western blotting to detect the expression of pyroptosis-related proteins: NLRP3, GSDMD-N, Caspase-1, IL-1β, IL-18, and ASC in flap tissues of the control, TQ, and TQ+EX527 groups. (B) Quantitative analysis of pyroptosis-related protein blots. (C) GSDMD-N protein expression levels in flap tissues of rats in the control, TQ, and TQ+EX527 groups measured by immunofluorescence (scale bar: 20 µm). (D) Fluorescence intensity of GSDMD-N expression in each group analyzed using Image J. (E) Western blotting to detect the expression of oxidative stress-related proteins: eNOS, HO1, and SOD1 in flap tissues of the control, TQ, and TQ+EX527 groups. (F) Quantitative analysis of oxidative stress-related protein blots. (G) Western blotting to detect the expression of apoptosis-related proteins: Bax, C-CASP3, and Bcl-2 in flap tissues of the control, TQ, and TQ+EX527 groups. (H) Quantitative analysis of apoptosis-related protein blots. Significance: *p < 0.05 and **p < 0.01. Data are presented as mean ± SEM, with five rats per group.

Article Snippet: Anti-cadherin 5 is provided by Wuhan Boster Biological Technology, Ltd. Primary antibodies against GAPDH, HO1, SOD1, Bax, Bcl-2, GSDMD-N, and VEGF are sourced from The Proteintech Group (Chicago, USA).

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Fluorescence

Journal: Cell reports

Article Title: Selenium Deficiency Is Associated with Pro-longevity Mechanisms

doi: 10.1016/j.celrep.2019.05.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-HO-1 , Novus Biologicals , NBP1-31341.

Techniques: Recombinant, Expressing, Purification, Sequencing, RNA sequencing, Software

Journal: Experimental eye research

Article Title: An inducible form of Nrf2 confers enhanced protection against acute oxidative stresses in RPE cells

doi: 10.1016/j.exer.2017.08.001

Figure Lengend Snippet: Constitutive and inducible Nrf2 expression results in increased phase II and other oxidative stress response genes. (A, B) ARPE-19 cells constitutively expressing FLAG-tagged (FT) Nrf2 (FT cNrf2) were assessed for canonical downstream Nrf2 genes using TaqMan probes (A) or western blotting (B). Primary antibodies were as follows: Nrf2 (Proteintech, Chicago, IL), NQO1 (Santa Cruz, Dallas, TX), HO1 (Bethyl Laboratories, Montgomery, TX), GCLC (Proteintech), GAPDH (Santa Cruz), and matrin-3 (Bethyl Laboratories). Nrf2 and KEAP1 (see panel ‘E’) were imaged on a LI-COR Odyssey Fc (LI-COR, Lincoln, NE) using either SuperSignal West Femto substrate (Thermo Fisher Scientific) or an IRDye secondary antibody (LI-COR, Lincoln, NE), while remaining proteins were detected solely using IRDye secondary antibodies and imaged on a LI-COR CLx (LI-COR). (C, D) ARPE-19 cells expressing inducible FT Nrf2 (FT iNrf2) were also assayed by qPCR (C) and western blotting (D). Dotted line in panel ‘C’ represents FT cNrf2 levels, as shown in panel ‘A’. (E, F) KEAP1 protein levels and KEAP1/CUL3/RBX1 ubiquitin ligase complex transcripts are not changed in FT cNrf2 or FT iNrf2 samples. KEAP1 primary antibody was from Santa Cruz. (G) Low levels of dox (100 ng/mL-1 μg/mL) for 96 h do not perturb mitonuclear protein balance. OXPHOS antibody cocktail (Abcam, Cambridge, MA), β-actin (LI-COR)). For qPCR experiments, n = 3, mean ± S.D., *p < 0.05, **p < 0.01, one sample t-test against a hypothetical mean of 1 (i.e., unchanged). Representative data of ≥3 independent experiments for all western blots, mean ± S.D. **p < 0.01, unpaired Student’s t-test assuming equal variance amongst samples.

Article Snippet: Primary antibodies were as follows: Nrf2 (Proteintech, Chicago, IL), NQO1 (Santa Cruz, Dallas, TX), HO1 (Bethyl Laboratories, Montgomery, TX), GCLC (Proteintech), GAPDH (Santa Cruz), and matrin-3 (Bethyl Laboratories).

Techniques: Expressing, Western Blot, Ubiquitin Proteomics